What is xho 1?

XhoI is a restriction endonuclease enzyme that recognizes the DNA sequence CTCGAG and cleaves between the C and G nucleotides. This enzyme is commonly used in molecular biology research to cut and manipulate DNA sequences for various applications such as cloning, sequencing, and gene expression analysis. XhoI is derived from the bacteria Xanthomonas holcicola and is classified as a type II restriction enzyme. It is commonly found in commercial restriction enzyme mixtures and is used in combination with other enzymes to create unique DNA cuts for specific research needs.